タイトル候補

D-Arabinose: A novel bioactive rare sugar found by a C. elegans growth assay

D-Arabinose: A novel bioactive rare sugar found by a  growth assay using Caenorhabditis elegans larvae.

D-Arabinose: A novel bioactive rare sugar discovered by a  growth assay using Caenorhabditis elegans larvae.

D-Arabinose: A novel bioactive rare sugar found by a  growth assay using Caenorhabditis elegans larvae.

Discovery of D-Arabinose as a novel bioactive rare sugar by a Caenorhabditis elegans growth assay.

D-Arabinose as a growth inhibior 

Analysis of lipofuscin

Analysis of lipofuscin

Ishii 1994 :  exitation 365nm, emission not filtered

Nishikawa 2013: DsRED,  exitation 510-560nm, 

emission 590-650nm

線虫マテメソ

To control for potential maternal effects of temperature, N2 and CC1 were allowed to grow for three generations at 15°, 20° or 25°C prior to the onset of these studies.

タイトル Comparison of the ～ activityなど

1.  In vitro Comparison of the Antimicrobial Activity of Ten Herbal Extracts Against Streptococcus mutans with Chlorhexidine.

2.  Comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens.

3.  Comparison of the growth inhibitory effects of canine IFN-alpha, -beta and -gamma on canine cells infected with Neospora caninum tachyzoites.

DR in axenic medium

J Gerontol A Biol Sci Med Sci. 2008 Mar;63(3):242-52.

Dietary restriction of Caenorhabditis elegans by axenic culture reflects nutritional requirement for constituents provided by metabolically active microbes.

Lenaerts I1, Walker GA, Van Hoorebeke L, Gems D, Vanfleteren JR.

Abstract

In Caenorhabditis elegans, several manipulations that affect nutrition slow development, reduce fecundity, and increase life span. These are viewed as dietary restriction (DR) and include culture in semidefined, nutrient-rich liquid medium that is axenic (i.e., there is no microbial food source). Here we describe convenient ways to exert DR by culture on agar plates containing axenic medium. We used these to explore whether effects of axenic culture really reflect DR. Our results imply that major nutrient components of axenic medium, and overall caloric content, are not limiting for life span. However, adding growth-arrested Escherichia coli as an additional food source rescued the effects of axenic culture. We then sought to identify the component of E. coli that is critical for normal C. elegans nutrition using add-back experiments. Our results suggest that C. elegans has a nutritional requirement for live, metabolically active microbes or, possibly, an unidentified, heat-labile, nonsoluble component present in live microbes.

LD50

中央有効値（ED50,LD50)

　生物の反応には，生きるか死ぬか，反応を起こすか起こさないかといった，質的なもの，不連続なものがあり，これは quantal response あるいは all or none type response とよばれている。このような反応に対しては，生物の50％が反応を示す量，中央有効値（ED50）あるいは50％致死量（LD50）が計算される。

「畜産を中心とする実験計画法」吉田　実（1975）　ｐ374

　成長阻害のIC50値はこの計算でよいのだろうか？

エピマー

D-All and L-Ido are the C3 and C5 epimers of D-Glc, respectively, and D-Tal is the C2 epimer of D-Gal or the C4 epimer of D-Man.

D-Ara は D-Rib のC2エピマーである。

Fフッ素の特性

1.サイズが小さいので，水素のミミック効果

2.電気陰性度大，静電的因子

3.C-F結合の結合エネルギー大，代謝されにくい

4.H→Fで疎水性の変化，体内動態に影響

書いていて，よくわからないものもあるが。
つまり，分子のサイズはあまり変化しないが，電気的な性質が大きく変化するということ？

希少糖アンチエイジングの特許性

　希少糖処理
→1.血糖値が下がる，脂肪が落ちる，がん細胞の増殖抑制する
→2.加齢性疾患のリスクが下がる
→3.結果としてアンチエイジング

すでにプシコース，アロースは1の特許が出ているので，3に新規性はない。

BBBのNote

BBBのNoteへ投稿したら，Regular paper で出せない理由をcover letterで書けと言われて返された。一応，日本語では書いていたのだが，英語で書けと言うことか。

cover letterか...ちゃんと書いたことがない（汗）大事だとは言うが...